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A comprehensive panel of primary antibodies was used to detect various proteins: FOXO4, p53, Ser15-phospho-p53, p21 (cyclin-dependent kinase inhibitor 1A), p16 (cyclin-dependent kinase inhibitor 2A), 3β-HSD, CYP11A1, CYP17A1 (cytochrome P450 family 17 subfamily A member 1), and several senescence-associated secretory phenotype (SASP) factors including IL-1α, IL-1β, IL-6, IL-10, TNF-α, and TGF-β

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[1] In adults with confirmed GH deficiency, sermorelin may also be considered as a treatment option, although its use in healthy older adults for “anti-aging” purposes remains unproven and is not an FDA-approved indication